3P23
Crystal structure of the Human kinase and RNase domains in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 85 |
| Detector technology | CCD |
| Collection date | 2009-05-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 109.850, 83.200, 116.080 |
| Unit cell angles | 90.00, 94.79, 90.00 |
Refinement procedure
| Resolution | 54.733 - 2.700 |
| R-factor | 0.2384 |
| Rwork | 0.235 |
| R-free | 0.29440 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.914 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.800 | 2.740 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 56524 | |
| <I/σ(I)> | 7.9 | 3.2 |
| Completeness [%] | 98.5 | 98.5 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 15% PEG 3350, 0.2M Ammonium Sulfate, pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
