3P23
Crystal structure of the Human kinase and RNase domains in complex with ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 85 |
Detector technology | CCD |
Collection date | 2009-05-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.92 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 109.850, 83.200, 116.080 |
Unit cell angles | 90.00, 94.79, 90.00 |
Refinement procedure
Resolution | 54.733 - 2.700 |
R-factor | 0.2384 |
Rwork | 0.235 |
R-free | 0.29440 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.914 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.800 | 2.740 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 56524 | |
<I/σ(I)> | 7.9 | 3.2 |
Completeness [%] | 98.5 | 98.5 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 15% PEG 3350, 0.2M Ammonium Sulfate, pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |