3OQ9

Structure of the FAS/FADD death domain assembly

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Detector technology	CCD
Collection date	2006-04-13
Wavelength(s)	0.9791
Spacegroup name	C 2 2 21
Unit cell lengths	135.204, 144.447, 131.574
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 6.800
Rwork	0.343
R-free	0.35420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.722
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.1.4)
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	7.040
High resolution limit [Å]	6.800	14.540	6.800
Rmerge	0.070	0.045	0.462
Number of reflections	2175
<I/σ(I)>	15
Completeness [%]	92.6	93.9	69.3
Redundancy	4.9	4.4	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	8.5	298	0.1 M Tris pH 8.5, 100 mM MgCl2, 5 % glycerol and 6-10 % PEG4000, hanging drop, temperature 298K