3OQ3

Structural Basis of Type-I Interferon Sequestration by a Poxvirus Decoy Receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Detector	NOIR-1
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	48.840, 75.900, 182.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.072 - 2.100
R-factor	0.2044
Rwork	0.203
R-free	0.23490
Structure solution method	SAD/MR
Starting model (for MR)	1itf
RMSD bond length	0.007
RMSD bond angle	1.003
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	SHARP
Refinement software	PHENIX (1.6.2_432)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.680	45.680	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.086	0.052	0.335
Total number of observations		27831	23035
Number of reflections	66438
<I/σ(I)>	6.7	15	2.5
Completeness [%]	95.9	99.3	92.5
Redundancy	3.29	3.62	3.24

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.3	293	17% 2-methyl-2,4-pentanediol (MPD), 2% polyethylene glycol 6000 (PEG 6000), and 100 mM NaOAc (pH 5.3), VAPOR DIFFUSION, HANGING DROP, temperature 293K