3OOI
Crystal Structure of Human Histone-Lysine N-methyltransferase NSD1 SET domain in Complex with S-adenosyl-L-methionine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-09 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.8103 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.875, 67.751, 69.086 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.610 - 1.750 |
R-factor | 0.16514 |
Rwork | 0.164 |
R-free | 0.18639 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.196 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.810 |
High resolution limit [Å] | 1.750 | 3.770 | 1.750 |
Rmerge | 0.057 | 0.428 | |
Number of reflections | 31587 | ||
<I/σ(I)> | 27 | 4.8 | |
Completeness [%] | 99.7 | 97.9 | 100 |
Redundancy | 5.7 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2M lithium sulfate, 0.1M HEPES, 25% PEG3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2M lithium sulfate, 0.1M HEPES, 25% PEG3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |