3OMH
Crystal structure of PTPN22 in complex with SKAP-HOM pTyr75 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2009-12-17 |
| Detector | APEX II CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 115.229, 46.707, 121.493 |
| Unit cell angles | 90.00, 101.43, 90.00 |
Refinement procedure
| Resolution | 36.752 - 2.900 |
| R-factor | 0.231 |
| Rwork | 0.229 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.510 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASES |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 3.000 |
| High resolution limit [Å] | 2.898 | 2.898 |
| Rmerge | 0.091 | |
| Number of reflections | 33348 | |
| Completeness [%] | 91.6 | 74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 293 | 20% PEG 3350, 0.1M Tris-Hcl, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
