3OLR
PTPN22 in complex with consensus phospho-tyrosine peptide 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2009-12-17 |
Detector | APEX II CCD |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 |
Unit cell lengths | 45.897, 62.766, 117.461 |
Unit cell angles | 99.06, 96.53, 105.06 |
Refinement procedure
Resolution | 50.000 - 2.500 |
Rwork | 0.162 |
R-free | 0.21010 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.227 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASES |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.087 | |
Number of reflections | 40826 | |
Completeness [%] | 97.6 | 76.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20% PEG 3350, 0.1M Tris-Hcl, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |