3OJM
Crystal Structure of FGF1 complexed with the ectodomain of FGFR2b harboring P253R Apert mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2002-10-31 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.92018 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.740, 72.349, 91.912 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.100 |
| Rwork | 0.229 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nun |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.350 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
| Number of reflections | 27015 | ||
| <I/σ(I)> | 40.5 | 105.4 | 34.8 |
| Completeness [%] | 99.5 | 99.1 | 98.6 |
| Redundancy | 12.2 | 13.1 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M Hepes, 22% monomethyl ether PEG5000, 0.2M ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
