3OGQ
Crystal Structure of 6s-98S FIV Protease with Lopinavir bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-22 |
| Detector | MARmosaic325 |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 31 |
| Unit cell lengths | 81.790, 81.790, 33.940 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 70.840 - 1.800 |
| R-factor | 0.2276 |
| Rwork | 0.225 |
| R-free | 0.27940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.408 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.840 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.051 | 0.401 |
| Number of reflections | 21978 | |
| <I/σ(I)> | 10.9 | 2 |
| Completeness [%] | 93.5 | 84.9 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | VAPOR DIFFUSION, HANGING DROP, temperature 277K |
