3OG8
Crystal structure of human RIG-I CTD bound to a 14-bp blunt-ended dsRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 173 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-04-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.990, 70.300, 125.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.960 - 2.400 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.320 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.960 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.086 | 0.448 |
| Number of reflections | 13384 | |
| <I/σ(I)> | 29.5 | 5.3 |
| Completeness [%] | 99.1 | 98 |
| Redundancy | 6 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 27% PEG MME 550, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
