3OG6

The crystal structure of human interferon lambda 1 complexed with its high affinity receptor in space group P212121

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-30
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	64.960, 85.790, 116.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.393 - 2.097
R-factor	0.1849
Rwork	0.184
R-free	0.22780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3HHC and PDB entry 3G9V
RMSD bond length	0.014
RMSD bond angle	1.226
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.070	0.034	0.840
Number of reflections	37819
<I/σ(I)>	10.5
Completeness [%]	97.1	99.9	79.6
Redundancy	6.8	7	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9	298	20% MPEG 2000, 100 mM Tris-HCl pH 7.9, 200 mM trimethyamine N-oxide dehydrate, VAPOR DIFFUSION, HANGING DROP, temperature 298K