3OG4
The crystal structure of human interferon lambda 1 complexed with its high affinity receptor in space group P21212
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-30 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 130.190, 65.440, 73.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.112 - 2.160 |
| R-factor | 0.1989 |
| Rwork | 0.198 |
| R-free | 0.24020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3HHC and PDB entry 3G9V |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.057 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.240 |
| High resolution limit [Å] | 2.160 | 4.650 | 2.160 |
| Rmerge | 0.057 | 0.041 | 0.710 |
| Number of reflections | 33364 | ||
| <I/σ(I)> | 11.2 | ||
| Completeness [%] | 97.4 | 97.9 | 81.7 |
| Redundancy | 3.7 | 3.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | 17% PEG 3350, 100 mM HEPES pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
