3OFI
Crystal structure of human insulin-degrading enzyme in complex with ubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 65 |
Unit cell lengths | 262.943, 262.943, 90.968 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.350 |
R-factor | 0.21116 |
Rwork | 0.210 |
R-free | 0.23983 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g47 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.430 |
High resolution limit [Å] | 2.350 | 5.060 | 2.350 |
Rmerge | 0.162 | 0.062 | 0.533 |
Number of reflections | 148306 | ||
<I/σ(I)> | 8.2 | ||
Completeness [%] | 100.0 | 99.8 | 99.8 |
Redundancy | 5.6 | 6.2 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 10-13% PEG MME 5000, 100 MM HEPES PH 7.0. 4-14% Tacsimate, 10% Dioxane, vapor diffusion, hanging drop, temperature 291K |