3OEF

Crystal structure of Y323F inactive mutant of p38alpha MAP kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-09-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.93
Spacegroup name	P 21 21 21
Unit cell lengths	64.734, 69.070, 74.409
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.535 - 1.600
R-factor	0.1845
Rwork	0.183
R-free	0.22080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.368
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: dev_392))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Number of reflections	44779
Completeness [%]	99.9	99.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.25	277	17% (w/v) PEG 3350, 0.1M Hepes pH 7.25, 0.2M KF, and 25mM b-OG, VAPOR DIFFUSION, SITTING DROP, temperature 277K