3OB1
Crystal structure of c-Cbl TKB domain in complex with double phosphorylated Spry2 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER PROTEUM X8 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2009-06-14 |
| Detector | BRUKER AXS MICROSTAR |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 6 |
| Unit cell lengths | 122.092, 122.092, 55.689 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.270 - 2.200 |
| R-factor | 0.18303 |
| Rwork | 0.181 |
| R-free | 0.22296 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bum |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.736 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.090 |
| High resolution limit [Å] | 2.020 | 4.350 | 2.020 |
| Rmerge | 0.091 | 0.051 | 0.460 |
| Number of reflections | 31335 | ||
| <I/σ(I)> | 13.9 | ||
| Completeness [%] | 99.5 | 100 | 98.1 |
| Redundancy | 9.2 | 16.6 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20% PEG 3350, 0.2M K/Na tartrate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
