3O4O

Crystal structure of an Interleukin-1 receptor complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	44.830, 177.470, 180.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.585 - 3.300
R-factor	0.2534
Rwork	0.252
R-free	0.28920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2i1b
RMSD bond length	0.012
RMSD bond angle	1.120
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.000	3.480
High resolution limit [Å]	3.300	3.300
Number of reflections	22655
Completeness [%]	99.8	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	17% PEG 10000, 0.1M Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K