3NX0
Hinge-loop mutation can be used to control 3D domain swapping and amyloidogenesis of human cystatin C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, Hamburg |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-28 |
| Wavelength(s) | 0.81272 |
| Spacegroup name | P 61 |
| Unit cell lengths | 76.316, 76.316, 97.723 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.040 |
| R-factor | 0.18137 |
| Rwork | 0.179 |
| R-free | 0.22474 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.836 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.110 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.078 | 0.429 |
| Number of reflections | 20617 | |
| <I/σ(I)> | 20.5 | 2.1 |
| Completeness [%] | 95.8 | 71.3 |
| Redundancy | 6.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 0.1 M sodium cacodylate, 0.2 M ammonium sulfate and 25% PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
