3NVK
Structural basis for substrate placement by an archaeal box C/D ribonucleoprotein particle
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MAR scanner 300 mm plate |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 330.039, 94.548, 97.729 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.690 - 3.209 |
R-factor | 0.2361 |
Rwork | 0.230 |
R-free | 0.28780 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.622 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 42641 | |
Completeness [%] | 83.3 | 83.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 303 | 400 mM potassium chloride, 200 mM-1.5 M sodium chloride, 150-250 mM magnesium acetate, 200 mM ammonium acetate, 50 mM HEPES-NaOH., pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 303 | 400 mM potassium chloride, 200 mM-1.5 M sodium chloride, 150-250 mM magnesium acetate, 200 mM ammonium acetate, 50 mM HEPES-NaOH., pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 303K |