3NAI
Crystal structures and functional analysis of murine norovirus RNA-dependent RNA polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.239 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.246, 196.562, 109.598 |
| Unit cell angles | 90.00, 114.81, 90.00 |
Refinement procedure
| Resolution | 39.530 - 2.560 |
| R-factor | 0.206 |
| Rwork | 0.203 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.254 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.144 | 0.460 |
| Number of reflections | 74150 | |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 98.7 | 95 |
| Redundancy | 5.3 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 1 M (NH4)2SO4, 100mM cacodylate (pH 6.5) , VAPOR DIFFUSION, HANGING DROP, temperature 277K |
