3MXM
TREX1 3' Exonuclease V201D Aicardi-Goutieres Syndrome Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.468, 86.026, 92.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.706 - 1.750 |
| R-factor | 0.1731 |
| Rwork | 0.172 |
| R-free | 0.20330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.112 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.4_113)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.068 | 0.240 |
| Number of reflections | 57078 | |
| <I/σ(I)> | 11.9 | 3.4 |
| Completeness [%] | 99.0 | 91.2 |
| Redundancy | 5.67 | 2.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 303 | 16% PEG 3350 0.1 M NaI 5% 1,4-butanediol, VAPOR DIFFUSION, SITTING DROP, temperature 303K |
