3MUG
Crystal structure of human Fab PG16, a broadly reactive and potent HIV-1 neutralizing antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 128.770, 66.250, 198.700 |
| Unit cell angles | 90.00, 98.00, 90.00 |
Refinement procedure
| Resolution | 24.970 - 2.490 |
| R-factor | 0.2094 |
| Rwork | 0.207 |
| R-free | 0.24850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7fab |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.250 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.970 | 2.620 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Number of reflections | 110752 | |
| <I/σ(I)> | 7.5 | 2.4 |
| Completeness [%] | 94.7 | 85.9 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 277 | 40% PEG 400, 0.08M sodium chloride, 0.1M CHES, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
