3ME2

Crystal structure of mouse RANKL-RANK complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2007-07-14
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9395
Spacegroup name	P 63
Unit cell lengths	121.225, 121.225, 94.995
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.800
R-factor	0.17541
Rwork	0.174
R-free	0.20504
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jtz
RMSD bond length	0.007
RMSD bond angle	0.999
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.191	0.995
Number of reflections	18587
<I/σ(I)>	18.6	2.9
Completeness [%]	100.0	100
Redundancy	16.3	13.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	294	0.1M sodium di-hydrogen phosphate, 2M sodium chloride, 0.1M potassium di-hydrogen phosphate, 0.1M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K
1	VAPOR DIFFUSION, SITTING DROP	6.5	294	0.1M sodium di-hydrogen phosphate, 2M sodium chloride, 0.1M potassium di-hydrogen phosphate, 0.1M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K