3MBW
Crystal structure of the human ephrin A2 LBD and CRD domains in complex with ephrin A1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-10 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 58.265, 215.787, 107.263 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.100 - 2.810 |
R-factor | 0.2286 |
Rwork | 0.226 |
R-free | 0.27230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 3C8X 1shw |
RMSD bond length | 0.009 |
RMSD bond angle | 1.150 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 16953 | |
<I/σ(I)> | 23.65 | 2.17 |
Completeness [%] | 99.7 | 100 |
Redundancy | 5.3 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 290.9 | 10.0% PEG 3350, 0.16M AMMONIUM PHOSPHATE, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290.9K |