3MA7
Crystal structure of Cardiolipin bound to mouse CD1D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-21 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.773, 50.921, 103.165 |
| Unit cell angles | 102.15, 92.22, 106.21 |
Refinement procedure
| Resolution | 44.680 - 2.290 |
| R-factor | 0.234 |
| Rwork | 0.232 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2akr |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.484 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.091 | 0.384 |
| Number of reflections | 37815 | |
| <I/σ(I)> | 12.9 | 1.9 |
| Completeness [%] | 95.4 | 88.4 |
| Redundancy | 1.9 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% POLYETHYLENE GLYCOL 4000, 300MM CALCIUM ACETATE, 6% ETHYLENE GLYCOL, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 295K |
