3MA7
Crystal structure of Cardiolipin bound to mouse CD1D
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-21 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 1 |
Unit cell lengths | 46.773, 50.921, 103.165 |
Unit cell angles | 102.15, 92.22, 106.21 |
Refinement procedure
Resolution | 44.680 - 2.290 |
R-factor | 0.234 |
Rwork | 0.232 |
R-free | 0.27300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2akr |
RMSD bond length | 0.013 |
RMSD bond angle | 1.484 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.290 | 2.290 |
Rmerge | 0.091 | 0.384 |
Number of reflections | 37815 | |
<I/σ(I)> | 12.9 | 1.9 |
Completeness [%] | 95.4 | 88.4 |
Redundancy | 1.9 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 20% POLYETHYLENE GLYCOL 4000, 300MM CALCIUM ACETATE, 6% ETHYLENE GLYCOL, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 295K |