3M4C
A Zn-mediated tetrahedral protein lattice cage encapsulating a microperoxidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795 |
Spacegroup name | H 3 2 |
Unit cell lengths | 126.892, 126.892, 167.944 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.660 - 1.900 |
R-factor | 0.19 |
Rwork | 0.187 |
R-free | 0.22300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.956 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.25) |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 91.894 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.063 | 0.308 |
Total number of observations | 42171 | |
Number of reflections | 41019 | |
<I/σ(I)> | 15.2 | 2.5 |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 12% PEG 1900 MME , 0.1 M HEPES pH 7.5 , 0.2 M NaCl, 2 mM ZnCl2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |