3M0C
The X-ray Crystal Structure of PCSK9 in Complex with the LDL receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9775 |
| Spacegroup name | P 65 |
| Unit cell lengths | 322.906, 322.906, 76.733 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 279.640 - 7.010 |
| R-factor | 0.342 |
| Rwork | 0.341 |
| R-free | 0.36200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 2QTW 1ijq |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.286 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 279.645 | 7.380 |
| High resolution limit [Å] | 7.010 | 7.010 |
| Rmerge | 0.173 | 0.776 |
| Number of reflections | 6162 | |
| <I/σ(I)> | 3.2 | 0.6 |
| Completeness [%] | 99.1 | 98.2 |
| Redundancy | 5.8 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 297 | 1.4 M Ammonium phosphate, 0.2M sodium chloride in 0.1M Imidazole buffer, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
