3LTF
Crystal Structure of the Drosophila Epidermal Growth Factor Receptor ectodomain in complex with Spitz
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-03 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.91790 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 118.208, 124.239, 186.508 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.200 |
| R-factor | 0.2405 |
| Rwork | 0.237 |
| R-free | 0.27542 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | dEGFR domains I-IV (pdb entry 3I2T) |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.126 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.500 | 3.340 |
| High resolution limit [Å] | 3.140 | 3.200 |
| Number of reflections | 45880 | |
| <I/σ(I)> | 10.8 | 2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.1 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 294 | 1.5 M sodium potassium phosphate, pH 6.9 4% tert-butanol , VAPOR DIFFUSION, HANGING DROP, temperature 294K |
