3LMS
Structure of human activated thrombin-activatable fibrinolysis inhibitor, TAFIa, in complex with tick-derived funnelin inhibitor, TCI.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-01-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0723 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 157.180, 157.180, 57.070 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.180 - 2.500 |
| R-factor | 0.18584 |
| Rwork | 0.185 |
| R-free | 0.21618 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d4u |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.280 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0046) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.200 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.109 | 0.628 |
| Number of reflections | 28241 | |
| <I/σ(I)> | 11.4 | 3.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.8 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2M KBr, 0.1M cacodylate, 15% PEG 4000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
