3LK9
DNA polymerase beta with a gapped DNA substrate and dTMP(CF2)P(CF2)P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-21 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.960, 79.800, 55.050 |
| Unit cell angles | 90.00, 108.46, 90.00 |
Refinement procedure
| Resolution | 24.280 - 2.500 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fms |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.072 | 0.493 |
| Number of reflections | 14278 | |
| <I/σ(I)> | 13.1 | 2.5 |
| Completeness [%] | 99.8 | |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 50 MM IMIDAZOLE,PH 7.5, SODIUM ACETATE 350 MM, PEG3350 16-18%, , VAPOR DIFFUSION, SITTING DROP, temperature 291K |
