3LDW
Crystal Structure of Plasmodium vivax geranylgeranylpyrophosphate synthase PVX_092040 with zoledronate and IPP bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-05 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | .91790 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 111.264, 139.543, 109.711 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.240 - 2.470 |
| R-factor | 0.2414 |
| Rwork | 0.239 |
| R-free | 0.28610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.540 |
| High resolution limit [Å] | 2.470 | 2.500 |
| Rmerge | 0.174 | 0.908 |
| Number of reflections | 60963 | |
| <I/σ(I)> | 13.03 | 2.03 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.3 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 20 % PEG3350 0.2 M LI2SO4 0.1 M TRIS 8.5 5% GLYCEROL,, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
