3LDB

Structure of JMJD6 complexd with ALPHA-KETOGLUTARATE and Fab Fragment.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-10
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	138.381, 138.381, 183.622
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.280 - 2.700
R-factor	0.25
Rwork	0.250
R-free	0.28500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 3LD8 JMJD6 and Fab Fragment
RMSD bond length	0.009
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	99.000
High resolution limit [Å]	2.610
Number of reflections	48696
Completeness [%]	89.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	277	0.1 M Bis(2-hydroxyethyl)-amino-tris(hydroxymethyl)-methane, 2.1 M Ammonium Sulphate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	5.6	277	0.1 M Bis(2-hydroxyethyl)-amino-tris(hydroxymethyl)-methane, 2.1 M Ammonium Sulphate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K