3LAW
Structure of GTP-bound L129F mutant Rab7
Experimental procedure
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 35.554, 89.335, 89.378 |
Unit cell angles | 71.24, 85.97, 85.91 |
Refinement procedure
Resolution | 19.880 - 2.800 |
R-factor | 0.23678 |
Rwork | 0.220 |
R-free | 0.27265 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.175 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.871 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 23869 | |
<I/σ(I)> | 7.7 | 1.7 |
Completeness [%] | 98.7 | 98.2 |
Redundancy | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 291 | 0.2 M sodium formate, 10mM trimethylamine hydrochloride, and 20% polyethylene glycol 3,350, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 291K |