3L8P

Crystal structure of cytoplasmic kinase domain of Tie2 complexed with inhibitor CEP11207

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2003-06-21
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97915
Spacegroup name	P 42 21 2
Unit cell lengths	85.602, 85.602, 107.344
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.170 - 2.400
R-factor	0.238
Rwork	0.238
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fvr
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.400
Rmerge	0.061
Number of reflections	16055
Completeness [%]	99.2
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	8% PEG 3350, 0.2M Tri-Ammonium Citrate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K