3L5H

Crystal structure of the full ectodomain of human gp130: New insights into the molecular assembly of receptor complexes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-28
Detector	ADSC QUANTUM 315
Wavelength(s)	0.95369
Spacegroup name	P 1 21 1
Unit cell lengths	89.001, 51.821, 167.157
Unit cell angles	90.00, 102.17, 90.00

Refinement procedure

Resolution	44.430 - 3.600
R-factor	0.2667
Rwork	0.263
R-free	0.33469
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	2.236
Data scaling software	SCALA (3.3.9)
Phasing software	PHASER
Refinement software	REFMAC (5.5.0066)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	163.400	3.790
High resolution limit [Å]	3.600	3.600
Rmerge		0.134
Number of reflections	17748
<I/σ(I)>		1.1
Completeness [%]	99.8
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	298	1.5-2.0M (NH4)2SO4, 0.1M imidazole-malonate buffer, pH 6.0, EVAPORATION, temperature 298K