3L2V
Crystal structure of the Prototype Foamy Virus (PFV) intasome in complex with manganese and MK0518 (Raltegravir)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 2009-10-13 |
Detector | ADSC |
Wavelength(s) | 0.9796 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 158.650, 158.650, 125.230 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.480 - 3.200 |
R-factor | 0.20643 |
Rwork | 0.205 |
R-free | 0.23204 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dlr |
RMSD bond length | 0.010 |
RMSD bond angle | 1.428 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.9) |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.123 | 3.370 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 26841 | |
Completeness [%] | 99.6 | 100 |
Redundancy | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 1.35M ammonium sulfate, 25% (v/v) glycerol, 4.8% (v/v) 1,6-hexanediol, 50mM Mes-NaOH, pH 6.5, vapor diffusion, hanging drop, temperature 291K |