3L26

Crystal structure of Zaire Ebola VP35 interferon inhibitory domain bound to 8 bp dsRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-12
Detector	NOIR-1
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	81.025, 81.025, 90.249
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.390 - 2.400
R-factor	0.1826
Rwork	0.179
R-free	0.25407
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fke
RMSD bond length	0.010
RMSD bond angle	1.215
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	REFMAC (5.3.0037)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.420
High resolution limit [Å]	2.400	2.400
Rmerge	0.150	0.876
Number of reflections	12297
<I/σ(I)>	21.3	3.8
Completeness [%]	100.0	100
Redundancy	14	13.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.7	293	2.9M SODIUM FORMATE, 0.1M SODIUM CITRATE, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	SODIUM FORMATE
2	1	1	SODIUM CITRATE
3	1	2	SODIUM FORMATE
4	1	2	SODIUM CITRATE