3KYM
Crystal structure of Li33 IgG2 di-Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 2007-12-04 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.97970 |
Spacegroup name | P 1 |
Unit cell lengths | 91.669, 109.545, 118.433 |
Unit cell angles | 61.46, 79.29, 87.59 |
Refinement procedure
Resolution | 19.960 - 2.620 |
R-factor | 0.26686 |
Rwork | 0.259 |
R-free | 0.33904 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3giz |
RMSD bond length | 0.012 |
RMSD bond angle | 1.615 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.620 | 2.620 |
Rmerge | 0.057 | 0.502 |
Number of reflections | 122107 | |
<I/σ(I)> | 16.8 | 1.6 |
Completeness [%] | 98.4 | 98.4 |
Redundancy | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 12% PEG3350, 0.1M phosphate citrate pH 4, 0.2M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K |