3KYM
Crystal structure of Li33 IgG2 di-Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-12-04 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.97970 |
| Spacegroup name | P 1 |
| Unit cell lengths | 91.669, 109.545, 118.433 |
| Unit cell angles | 61.46, 79.29, 87.59 |
Refinement procedure
| Resolution | 19.960 - 2.620 |
| R-factor | 0.26686 |
| Rwork | 0.259 |
| R-free | 0.33904 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3giz |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.615 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.620 | 2.620 |
| Rmerge | 0.057 | 0.502 |
| Number of reflections | 122107 | |
| <I/σ(I)> | 16.8 | 1.6 |
| Completeness [%] | 98.4 | 98.4 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 12% PEG3350, 0.1M phosphate citrate pH 4, 0.2M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
