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3KRX

Human GRK2 in complex with Gbetgamma subunits and balanol (co-crystal)

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 21-ID-G
Synchrotron siteAPS
Beamline21-ID-G
Temperature [K]150
Detector technologyIMAGE PLATE
Collection date2008-11-08
DetectorMAR scanner 300 mm plate
Spacegroup nameC 1 2 1
Unit cell lengths185.076, 73.290, 121.780
Unit cell angles90.00, 114.51, 90.00
Refinement procedure
Resolution25.000 - 3.100
R-factor0.232
Rwork0.230
R-free0.27000
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3cik
RMSD bond length0.006
RMSD bond angle0.912
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHASER
Refinement softwareREFMAC (5.5.0072)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]25.0003.200
High resolution limit [Å]3.1003.100
Number of reflections26894
<I/σ(I)>15.41.2
Completeness [%]99.298.8
Redundancy3.12.7
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1277Well contained 100 mM MES, pH 6.0, 200 mM NaCl, 9% PEG 3350. Drops were 1 uL protein, 1 uL well, VAPOR DIFFUSION, HANGING DROP, temperature 277K

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