3KRX
Human GRK2 in complex with Gbetgamma subunits and balanol (co-crystal)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 150 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-11-08 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 185.076, 73.290, 121.780 |
| Unit cell angles | 90.00, 114.51, 90.00 |
Refinement procedure
| Resolution | 25.000 - 3.100 |
| R-factor | 0.232 |
| Rwork | 0.230 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cik |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.912 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.200 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 26894 | |
| <I/σ(I)> | 15.4 | 1.2 |
| Completeness [%] | 99.2 | 98.8 |
| Redundancy | 3.1 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 277 | Well contained 100 mM MES, pH 6.0, 200 mM NaCl, 9% PEG 3350. Drops were 1 uL protein, 1 uL well, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
