3KNT
Crystal structure of Methanocaldococcus jannaschii 8-oxoguanine glycosylase/lyase in complex with 15mer DNA containing 8-oxoguanine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-15 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0332 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.800, 150.030, 90.270 |
Unit cell angles | 90.00, 107.53, 90.00 |
Refinement procedure
Resolution | 19.800 - 2.700 |
R-factor | 0.1901 |
Rwork | 0.188 |
R-free | 0.22510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fhf |
RMSD bond length | 0.004 |
RMSD bond angle | 0.984 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.800 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.124 | 0.010 |
Number of reflections | 37810 | |
<I/σ(I)> | 12.58 | 1.72 |
Completeness [%] | 99.0 | 99.2 |
Redundancy | 3.82 | 3.87 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | PEG-8000 16%, 0.1M NaCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |