3KJ4
Structure of rat Nogo receptor bound to 1D9 antagonist antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2004-03-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.990 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 188.664, 125.488, 90.575 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.040 - 3.100 |
R-factor | 0.25613 |
Rwork | 0.253 |
R-free | 0.31775 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ozn |
RMSD bond length | 0.025 |
RMSD bond angle | 2.509 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.210 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.151 | 0.637 |
Number of reflections | 37986 | |
<I/σ(I)> | 9.5 | 1.39 |
Completeness [%] | 99.2 | 98.4 |
Redundancy | 4.4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 14% Peg3350, 0.4M zinc acetate, 0.1M magnesium chloride, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |