3KGC

Isolated ligand binding domain dimer of GluA2 ionotropic glutamate receptor in complex with glutamate, LY 404187 and ZK 200775

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Detector technology	CCD
Collection date	2007-10-19
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	66.657, 95.586, 97.047
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.063 - 1.550
R-factor	0.1977
Rwork	0.196
R-free	0.22447
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ftl
RMSD bond length	0.009
RMSD bond angle	1.321
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.550
Number of reflections	81451

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	277	19% PEG 4000, 0.3 M ammonium sulfate, 0.1 M sodium acetate, pH 5.0, vapor diffusion, hanging drop, temperature 277K