3KGC
Isolated ligand binding domain dimer of GluA2 ionotropic glutamate receptor in complex with glutamate, LY 404187 and ZK 200775
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Detector technology | CCD |
Collection date | 2007-10-19 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.657, 95.586, 97.047 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.063 - 1.550 |
R-factor | 0.1977 |
Rwork | 0.196 |
R-free | 0.22447 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ftl |
RMSD bond length | 0.009 |
RMSD bond angle | 1.321 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.550 |
Number of reflections | 81451 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 19% PEG 4000, 0.3 M ammonium sulfate, 0.1 M sodium acetate, pH 5.0, vapor diffusion, hanging drop, temperature 277K |