3K8Y
Allosteric modulation of H-Ras GTPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-25 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 88.475, 88.475, 134.906 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 9.992 - 1.300 |
| R-factor | 0.201 |
| Rwork | 0.200 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rge |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.056 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.4_6) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.350 |
| High resolution limit [Å] | 1.290 | 1.290 |
| Rmerge | 0.100 | 0.857 |
| Number of reflections | 49586 | |
| <I/σ(I)> | 17.9 | 2.2 |
| Completeness [%] | 97.3 | 99.7 |
| Redundancy | 6.3 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291.15 | 0.2M Calcium acetate, 20% PEG 3350, 0.05% n-Octyl-B-D-glucopyranoside, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K |
