3K6N
Crystal structure of the S225E mutant Kir3.1 cytoplasmic pore domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-09-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 77.455, 77.455, 86.723 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.000 |
Rwork | 0.254 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1n9p |
RMSD bond length | 0.005 |
RMSD bond angle | 1.220 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.050 | 0.350 |
Number of reflections | 16877 | |
<I/σ(I)> | 39.8 | 7.8 |
Completeness [%] | 91.4 | 100 |
Redundancy | 11.2 | 10.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | sodium citrate, ammonium acetate, barium choride, MPD, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |