3K43
Crystal structure of sCD-MPR mutant E19Q/K137M pH 6.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 98 |
Detector technology | IMAGE PLATE |
Collection date | 2009-04-15 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | I 4 |
Unit cell lengths | 102.978, 102.978, 99.818 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.460 - 2.000 |
Rwork | 0.165 |
R-free | 0.19900 |
Structure solution method | difference fourier |
Starting model (for MR) | 3k42 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
Rmerge | 0.068 | 0.035 | 0.413 |
Number of reflections | 35223 | ||
<I/σ(I)> | 15.2 | ||
Completeness [%] | 99.7 | 97.9 | 100 |
Redundancy | 5.7 | 5.9 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 0.1M sodium cacodylate, 3M ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |