3K40
Crystal structure of Drosophila 3,4-dihydroxyphenylalanine decarboxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0908 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 105.809, 108.597, 86.294 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.880 - 1.750 |
| R-factor | 0.19811 |
| Rwork | 0.197 |
| R-free | 0.22600 |
| Structure solution method | MOLREP |
| Starting model (for MR) | 1js3 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.423 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.060 | 0.560 |
| Number of reflections | 100700 | |
| Redundancy | 15.1 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 15% PEG 4000, 20% glycerol, 200 mM CaCl2, and 100 mM Cacodylic acid, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
