3K3O
Crystal structure of the catalytic core domain of human PHF8 complexed with alpha-ketoglutarate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2009-06-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.092, 52.532, 134.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yu2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.103 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.050 | 0.524 |
| Number of reflections | 19197 | |
| <I/σ(I)> | 48.5 | 3.2 |
| Completeness [%] | 85.6 | 4.3 |
| Redundancy | 6.4 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | 200mM KCl, 100mM Mes, pH 6.5, 20% PEG5K, 5mM H3K9me2, 1.7mM KG, 1mM DTT, 1mM Fe2+, VAPOR DIFFUSION, temperature 298K |
