3JTC
Importance of Mg2+ in the Ca2+-Dependent Folding of the gamma-Carboxyglutamic Acid Domains of Vitamin K-Dependent clotting and anticlotting Proteins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-04-25 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.220, 62.360, 71.030 |
| Unit cell angles | 90.00, 101.81, 90.00 |
Refinement procedure
| Resolution | 23.220 - 1.600 |
| R-factor | 0.19246 |
| Rwork | 0.191 |
| R-free | 0.22648 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 1.450 |
| RMSD bond angle | 2.566 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.455 | |
| Number of reflections | 66375 | |
| <I/σ(I)> | 27 | 27 |
| Completeness [%] | 90.1 | 91 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | PEG 400, potassium chloride, magnesium chloride, calcium chloride, HEPES, PH 7.0, VAPOR DIFFUSION |
