3JBQ
Domain Organization and Conformational Plasticity of the G Protein Effector, PDE6
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
| Method | Applied 3 ul of sample per grid, blotted for 1 second before plunging |
EM Data Collection
| Microscope | JEOL 2010F |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2 |
| Energy Filter | FEI |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 11 |
| Software | PHENIX,UCSF Chimera,EMAN |
| Symmetry | C2 |
