3JB9
Cryo-EM structure of the yeast spliceosome at 3.6 angstrom resolution
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
| Method | Blot for 2.5 seconds before plunging |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 1.4 |
| Detector | GATAN K2 (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.6 |
| Software | RELION |
| Symmetry | C1 |
