3JAJ
Structure of the engaged state of the mammalian SRP-ribosome complex
This is a non-PDB format compatible entry.
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
Method | 3 uL of sample was incubated on the grid for 30 seconds before blotting for 3 seconds |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Detector | FEI FALCON II (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.75 |
Software | Coot,REFMAC,UCSF Chimera,RELION |
Symmetry | C1 |