3JAJ
Structure of the engaged state of the mammalian SRP-ribosome complex
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
| Method | 3 uL of sample was incubated on the grid for 30 seconds before blotting for 3 seconds |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON II (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.75 |
| Software | Coot,REFMAC,UCSF Chimera,RELION |
| Symmetry | C1 |
