3JAF
Structure of alpha-1 glycine receptor by single particle electron cryo-microscopy, glycine/ivermectin-bound state
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
| Method | Blot for 3.5 seconds before plunging. |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 0.01 |
| Energy Filter | GIF |
| Detector | GATAN K2 (4k x 4k) |
| Number of Images | 2489 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.8 |
| Software | UCSF Chimera,RELION |
| Symmetry | C5 |
